Reporter

Part:BBa_K1718001

Designed by: Naomi Iverson   Group: iGEM15_CU_Boulder   (2015-09-13)


pBAD promoter with RBS-RFP

To test the leakiness of the pBAD promoter, a plasmid must be assembled with a gene that indicates when the promoter is stimulated by L-arabinose. To do this, we chose Red Fluorescent Protein (RFP) to help signal when the promoter responds.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1791
    Illegal AgeI site found at 1903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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