Composite

Part:BBa_K1717000

Designed by: Our Lady of the Snows Catholic Academy 2015   Group: iGEM15_OLS_Canmore_AB_CA   (2015-09-13)

Keratinase A under control of LacI promoter.

Keratinases are proteolytic enzymes which break the disulphide bonds in various keratin substrates. This KERA expression cassette is a composite part comprised of an RBS + IPTG-inducible promoter (BBa_J04500) and a synthesized KERA-expressing gene, optimized for expression in E. coli and most active in degrading keratin in feathers. This part, when expressed in cells, can be tested for activity through looking for zones of clearing on skim milk agar plates where cells are growing, as well as by looking at degradation of keratin-containing substrates (hair, feathers, chemically synthesized keratin, etc.) when grown with cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 279
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None