Generator

Part:BBa_K1713017

Designed by: Keji Yan   Group: iGEM15_HZAU-China   (2015-09-14)

luxpR+B0034+AiiA(+LVA)+B0015

Expresses Aiia to hydrolyze AHL internal AHL. And the expression of Aiia is regulated by the concentration of AHL. Aiia is a kind of enzyme could hydrolyze AHL. The RBS of Aiia is B0034. The promote LuxPR is regulated by the concentration of AHL (N-Acyl homoserine lactone). LuxI's RBS is B0034, Aiia's RBS is B0032. LVA is degradation tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None