Coding
Part:BBa_K1711000
Designed by: Anastasia Nicolov Group: iGEM15_Washington (2015-09-17)
AFB2 auxin-binding F-box protein
This part, called AFB2, is an F-box protein that binds the common plant hormone auxin. F-box proteins contain an F-box domain that mediates degradation of other proteins by recognizing substrates for ubiquitination.
In our system, a dCas9-degron-repressor complex represses expression of lacZ. In the presence of auxin, the F-box protein and the presence of auxin, the AFB2 binds to auxin and the degron; the F-box protein then recruits the ubiquitin ligase to degrade the dCas9-degron-repressor complex. When this occurs, lacZ is no longer repressed and production of beta galactosidase occurs. Beta galactosidase acts as a reporter for the presence of auxin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 591
Illegal XbaI site found at 804
Illegal XbaI site found at 1242 - 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1204
Illegal XhoI site found at 1357 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 591
Illegal XbaI site found at 804
Illegal XbaI site found at 1242 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 591
Illegal XbaI site found at 804
Illegal XbaI site found at 1242 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 611
Illegal BsaI site found at 1306
Illegal SapI.rc site found at 307
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Categories
Parameters
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