Coding

Part:BBa_K1711000

Designed by: Anastasia Nicolov   Group: iGEM15_Washington   (2015-09-17)


AFB2 auxin-binding F-box protein

This part, called AFB2, is an F-box protein that binds the common plant hormone auxin. F-box proteins contain an F-box domain that mediates degradation of other proteins by recognizing substrates for ubiquitination.

In our system, a dCas9-degron-repressor complex represses expression of lacZ. In the presence of auxin, the F-box protein and the presence of auxin, the AFB2 binds to auxin and the degron; the F-box protein then recruits the ubiquitin ligase to degrade the dCas9-degron-repressor complex. When this occurs, lacZ is no longer repressed and production of beta galactosidase occurs. Beta galactosidase acts as a reporter for the presence of auxin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 591
    Illegal XbaI site found at 804
    Illegal XbaI site found at 1242
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1204
    Illegal XhoI site found at 1357
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 591
    Illegal XbaI site found at 804
    Illegal XbaI site found at 1242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 591
    Illegal XbaI site found at 804
    Illegal XbaI site found at 1242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 611
    Illegal BsaI site found at 1306
    Illegal SapI.rc site found at 307


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Categories
Parameters
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