Regulatory

Part:BBa_K1709006

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-18)


RBS-LuxI-His-RBS-LuxR-E-Term-Plux

Autoinducer synthetase for acyl-homoserine lactones (AHL): LuxI synthesizes AHL (3OC6HSL). Two molecules of AHL form a complex with two molecules of the LuxR protein. This complex then binds to a palyndromic site on the lux promoter, increasing its transcription rate. LuxI, LuxR and the right lux promoter are all derived from V. fischeri. In absence of AHL, the right Lux promotor only gives weak constitutive expression of downstream genes. Any gene you would like to be controlled by AHL and thus cell density can be cloned after this complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1427
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1502


[edit]
Categories
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//regulation/positive
Parameters
biologyV. Fischeri
controlluxR, HSL
directionForward
positive_regulators1
proteinluxI-His, LuxR-E