Part:BBa_K1707013

The translation of the thermosensitive cI repressor (cI857) is placed under the control of an RNA thermometer. This construction allows the controlled repression of the expression of a gene A with a cI promoter within the 30-35°C range of temperature. Below 30°C, the secondary structure formed by the RNA thermometer in the mRNA prevents the translation of cI857, A is therefore expressed. Above 30°C, the RNA thermometer is unfolded, allowing the translation of cI857. Under 35°, this later is properly folded and active, repressing the expression of A. Above 35°C, cI857 is under an inactive form and A is expressed.