Coding

Part:BBa_K1706002

Designed by: Chaohui Gao   Group: iGEM15_Jilin_China   (2015-09-18)


A Synthetic Formaldehyde Dehydrogenase Gene

PADH, formaldehyde dehydrogenase from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH. This is a synthetic formaldehyde dehydrogenase gene which codon were optimized using E.coli abundant codon. Amino acid sequence information reference NCBI YP_001751724.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 353
    Illegal XhoI site found at 800
    Illegal XhoI site found at 872
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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