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Part:BBa_K1705006

Designed by: Nathan Hunt   Group: iGEM15_MIT   (2015-09-18)

pLux

pLux-ON is a promoter activated by LuxR-AHL (there is also a version inhibited LuxR). It is used in quorum sensing systems: one organism expresses LuxI, which synthesizes AHL molecules. These diffuse freely into the surrounding environment. When an AHL enters a strain expressing LuxR, they bind, and LuxR-AHL activates pLux. pLux thus enables expression of a gene to be tied to surrounding concentration of other (LuxI-expressing) organisms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 164


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