Part:BBa_K1702004

trans-zeatin biosynthesis pathway

Biosynthesis pathway of trans-zeatin for E. coli. Trans-zeatin is a cytokinin which increases lateral bud growth, prevents leaf senescence, and increases cellular biomass of plants. Cytokinins function by signaling to activate proteins which increase transcription and cell division.

Synthesized tzs gene (from Agrobacterium tumefaciens C58) and LOG (Oryza satifa), both codon optimized for E. coli as a biobrick. The tzs gene encodes for the enzyme which converts 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate (HMBDP) into zeatin riboside 5'-phosphate. This reaction is AMP-dependent. The LOG gene produces N6-dimethylallyladenine by removing the phosphoribosyl group from N6-(delta2-isopentenyl)-adenosine 5'-monophosphate. Hydroxylation of the N6-dimethylallyladenine produces biologically-active trans-zeatin.

Trans-zeatin biosynthesis pathway. The tzs gene performs the same function as the tmr gene in the process shown.

Usage and Biology

This part was combined with a lac promoter and submitted to the registry. This composite part and its characterization can be seen here.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 702
  Illegal AgeI site found at 1074
  Illegal AgeI site found at 1082
  Illegal AgeI site found at 1238
  
• 1000
  COMPATIBLE WITH RFC[1000]