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Part:BBa_K1702003

Designed by: Olivia Smith   Group: iGEM15_CSU_Fort_Collins   (2015-09-17)


Lipase L1 from Bacillus stearothermophilus

This plasmid encodes for an extracellular lipase which breaks down triacylglycerols (TAGs), diacylglycerols (DAGs), and monoacylglycerols (MAGs) into free fatty acids and glycerol. L1 comes from Bacillus stearothermophilus, a thermophilic bacteria. Expression of this gene is under control of the lac promoter BBa_J04500, which is inducible with lactose or IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 707


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