Device

Part:BBa_K1698003

Designed by: Brandon Malone   Group: iGEM15_Cork_Ireland   (2015-09-18)

BaseHunter : Activated to become detector for 32bp target sequence on SRY Gene

Once digested with the four enzymes specified in our team's protocols, this part can be used to differenciate between male and female mammalian DNA. Further optimisation of part is needed for use in distinguishing male and female genomic DNA. Part is accurate in detecting target when isolated from a genomic sample. For protocol for construction of detector by digestion of plasmid see [http://2015.igem.org/Team:Cork_Ireland]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706
    Illegal SapI site found at 920


[edit]
Categories
Parameters
None