Tag

Part:BBa_K1696017

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-15)

tag3 for mf-lon to recognize

Mf-ssrA tag3 is one of the variants from mf-ssrA tag1 (BBa_K1696013) that could maintain steady-state RFP levels and display a range of mf-Lon-dependent degradation rates. Among all other tags, the proteolysis ability of this tag is a little bit weaker than mf-ssrA tag2 (BBa_K1696016) and mf-ssrA1 (BBa_K1696013) while stronger than the other variants.


Reference:

Cameron D E, Collins J J. Tunable protein degradation in bacteria.[J]. Nature Biotechnology, 2014, 32(12).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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