Composite

Part:BBa_K1696015

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-14)

Mf-ssrA Tag1 reporter

Mf-ssrA tag can be degraded by mf-Lon, which may bring plenty of applications in all kinds of fields. However, in order to implement a more practical and quick method for detection, we incorporated tag1 (BBa_K1696013) into a RFP-based test platform in E.coli. Once the protein has been expressed, it can lead to the red fluorescence detected. With the presence of mf-Lon, the red fluorescence can eventually drop off or even disappear.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None