Part:BBa_K1696012

Mf-lon Protease

Endogenous Lon protease (mf-Lon) is isolated from Gram-positive M. florum. It constructs a degradation system that does not rely on host degradation systems and can function in a wide range of bacteria[1]. The mf-Lon-regulated system updates the knowledge about types of regulation which not only contains genomic and transcriptional regulation, but proteomic regulation should be included as well. M.florum ssrA tag (mf-ssrA) is degraded by its endogenous Lon protease (mf-Lon) but not by E.coli Lon or ClpXP, and mf-Lon does not recognize or degrade ec-ssrA (E.coli ssrA tag) providing protease and cognate degradation tag with orthogonal functionality in E. coli[2].

Reference:

[1] Cameron D E, Collins J J. Tunable protein degradation in bacteria.[J]. Nature Biotechnology, 2014, 32(12).

[2] Gur E, Sauer RT: Evolution of the ssrA degradation tag in Mycoplasma: Specificity switch to a different protease. Proceedings of the National Academy of Sciences 2008, 105:16113–16118

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1834
  Illegal AgeI site found at 1918
  Illegal AgeI site found at 2124
  Illegal AgeI site found at 2149
  
• 1000
  COMPATIBLE WITH RFC[1000]