Part:BBa_K1696010

Riboflavin enhancing parts

We construct this part based on BBa_K169011 and BBa_K1172303.

Fig1.The design of this part.

Based on co-factor model, EET pathway points out that FMN plays a critical role in electron transfer. Through the study by Dr. Tao Chen, they have weakened the RBS upstream of ribC to divert more of the material flux to RF production. Based on their viewpoint, we got further to design a strong RBS sequence upstream of the ribC and we rename the new part as EC10*(BBa_K1696010). The yield of EC10* reached 90 mg/L(as shown in Figure2). The strong RBS before ribC sequence can lead to the FMN accumulation while riboflavin consumes a bit, which may in turn, result in increase both for riboflavin and FMN as a whole.

Figure 2. The yield of riboflavin in different strains: EC10(BBa_K1696011), Rf02S (Δpgi + EC10), EC10*(this part)

Figure 3. The yield of riboflavin in different strains: EC10, Rf02S (Δpgi + EC10), EC10*

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3386
  Illegal BamHI site found at 1370
  Illegal XhoI site found at 64
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 262
  Illegal AgeI site found at 992
  Illegal AgeI site found at 1034
  Illegal AgeI site found at 2752
  Illegal AgeI site found at 3139
  
• 1000
  COMPATIBLE WITH RFC[1000]