Composite

Part:BBa_K1696009

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-14)

Lactate sensing system

In the system, promoter P170 has been creatively constructed into E.coli to control the certain concentration of lactate. Besides, the activation of P170 cannot achieve without the help of rcfB and in order to measure the final result as convenient as possible, we also introduce a RFP reporter gene into the plasmid. Consequently, when acidification reaches a threshold, activated trans-acting protein RcfB ( BBa_K1696001) will bind to ACiD-box and lead to P170 activation. Thus, we can see the red fluorescence without any pretreatment. The characterization of this part was done using a P170-promoter + RFP construct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
    Illegal NheI site found at 1256
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 999
    Illegal AgeI site found at 1111
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1262
    Illegal BsaI.rc site found at 1990


[edit]
Categories
Parameters
None