Composite

Part:BBa_K1696004

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-11)

L-lactate producing part2


The NAD+-dependent L-lactate dehydrogenase gene from Geobacillus stearothermophilus is a promising enzyme for L-lactate mass production. In order to achieve even more lactate generation to feed exoelectrogen in our system, we choose a strong promoter and strong RBS to construct L-Lactate part. Considering that the strong promoter and RBS can ensure the over-expression of L-lactate, we believe that this kind of construction will lead to an increasing power generation and potential application in the future.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 36
    Illegal BsaI.rc site found at 1028


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Categories
Parameters
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