Composite

Part:BBa_K1695052

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-24)

pL8-UV5 + RBS Bacteriophage 21 Lysis Cassette S R Rz

The gene cassette encodes S protein (holin), R protein (endolysin) and Rz protein (spanin) from bacteriophage 21. The S gene does not contain the anti-holin sequence. R and Rz genes are wild type. Each gene is paired with the strong RBS BBa_B0034. The gene cassette is under the control of the strong pL8-UV5 constitutive promoter BBa_K1695000.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1986


[edit]
Categories
Parameters
None