Composite

Part:BBa_K1695049

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-17)

pL8-UV5 + Riboswitch Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz

The gene cassette encodes S protein (holin), R protein (endolysin) and Rz protein (spanin) from bacteriophage 21. All three genes in the cassette are codon optimized and paired with the strong RBS BBa_B0034 and a riboswitch for the regulation on its translation by theophylline. The gene cassette is preceded by the strong pL8-UV5 constitutive promoter BBa_K1695000.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1760
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1605
  • 1000
    COMPATIBLE WITH RFC[1000]


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