Composite

Part:BBa_K1695047

Designed by: Ma Lee   Group: iGEM15_CityU_HK   (2015-08-17)


pLacIQ + Riboswitch Bacteriophage λ Codon Optimized Lysis Cassette S R Rz

Bacteriophage Lambda Wild-type Holin, Endolysin, Spanin codon optimized with riboswitch to lyse the cells and with a lacI gene to repress the lysis cassette when the lactose is absent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1374
    Illegal AgeI site found at 1864
    Illegal AgeI site found at 2116
    Illegal AgeI site found at 2513
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None