Composite

Part:BBa_K1695045

Designed by: Ma Lee   Group: iGEM15_CityU_HK   (2015-08-17)

pL8-UV5 + RBS Bacteriophage λ Codon Optimized Lysis Cassette S mutant R Rz

The gene cassette encodes S protein (holin), R protein (endolysin) and Rz protein (spanin) from bacteriophage λ. All three genes in the cassette are codon optimized and are preceded by the strong RBS BBa_B0034. The gene cassette is under the control of the strong pL8-UV5 constitutive promoter BBa_K1695000. To shorten the lysis time, a missense mutation has been introduced in the S gene such that alanine (A) at position 50 is replaced by glycine (G).


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1824
  • 1000
    COMPATIBLE WITH RFC[1000]


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