Composite

Part:BBa_K1695044

Designed by: Ma Lee   Group: iGEM15_CityU_HK   (2015-08-17)


pLacIQ + RBS Bacteriophage λ Codon Optimized Lysis Cassette S R Rz

Bacteriophage Lambda Wild-type Holin, Endolysin, Spanin codon optimized with strong promoters to lyse the cells and lacI gene to repress the lysis cassette when the lactose is absent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1824
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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