Composite

Part:BBa_K1695041

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-17)

RBS Bacteriophage 21 Codon Optimized Lysis Cassette S mutant R Rz

The gene cassette encodes S protein (holin), R protein (endolysin) and Rz protein (spanin) from bacteriophage 21. All three genes in the cassette are codon optimized and are preceded by the strong RBS BBa_B0034. To shorten the lysis time, two mutations have been introduced to the S gene (Pang, Park and Young, 2010): (1) Deletion of TMD1 (2) Single point mutation such that serine at position 41 is replaced by asparagine.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 410
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None