Composite

Part:BBa_K1695037

Designed by: Ma Lee   Group: iGEM15_CityU_HK   (2015-08-13)

Riboswitch Bacteriophage λ Codon Optimized Lysis Cassette S R Rz

The gene cassette encodes S protein (holin), R protein (endolysin) and Rz protein (spanin) from bacteriophage λ. All three genes in the cassette are codon optimized and paired with the strong RBS BBa_B0034. A riboswitch precedes the gene cassette for the regulation on its translation by theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
    Illegal AgeI site found at 586
    Illegal AgeI site found at 838
    Illegal AgeI site found at 1235
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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