Translational_Unit

Part:BBa_K1695030

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-12)

RBS + Bacteriophage λ codon-optimized S mutant gene

The gene is codon optimized and encodes S protein (holin) from bacteriophage λ with the anti-holin sequence removed. It is preceded by the strong RBS BBa_B0034. The gene also contains a missense mutation such that alanine (A) at position 50 is replaced by glycine (G) in an otherwise wild type sequence.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None