Translational_Unit

Part:BBa_K1695008

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-11)

RBS + Bacteriophage 21 codon optimized S mutant gene

The gene codes for S protein (holin) from bacteriophage 21 with the anti-holin sequence removed. To shorten the lysis time, two mutations have been introduced to the otherwise wild-type gene (Pang, Park and Young, 2010):

(1) Deletion of TMD1

(2) Single point mutation such that serine at position 41 is replaced by asparagine.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None