Coding

Part:BBa_K1692006

Designed by: Daniel Kunin   Group: iGEM15_Stanford-Brown   (2015-08-03)

UbiX with T7 Promoter

UbiX with T7 promoter

UbiX is a flavin prenyltransferase that normally plays a role in ubiquinone biosynthesis in E. coli. UbiX transfers a prenyl group from dimethylallyl monophosphate (DMAP) to flavin mononucleotide (FMN), thereby creating a cofactor that happens to be essential to the functionality of FDC. This part is contains the protein-coding UbiX sequence from E. coli. Additionally, this part includes a T7 promoter, allowing for inducible expression. See our synthesized UbiX for more results.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
Parameters
None