Coding
ModLac

Part:BBa_K1688020

Designed by: Fredrik Lindeberg   Group: iGEM15_Uppsala   (2015-09-15)

ModLac laccase with His-tag and HlyA export tag

A modified laccase (D439A/M510L CueO) with N-His6 tag attached to it via a linker sequence and an export tag (HlyA, BBa_K554002) attached to its C-terminus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


Important: This part is not available in the registry yet, however, the same part is available from the registry with the standard RBS B0034: BBa_K1688006.

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Parameters
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