Translational_Unit
Part:BBa_K1688003
Designed by: Biosurfers Group: iGEM15_Uppsala (2015-09-15)
RhlB Gene
The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene (Ba_K1688002) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from rhlB gene connects the tail to a rhamnose molecule. The rhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomonas aeruginosa. The biobrick Ba_K1688003 is an assembly of RBS with BBa_K1331004 that codes for RhlB enzyme
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1164
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 57
Illegal NgoMIV site found at 778
Illegal NgoMIV site found at 891 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 407
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Categories
Parameters
n/a | RhlB Gene |