Translational_Unit

Part:BBa_K1688003

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)

RhlB Gene

The enzymes responsible for producing mono-rhamnolipids (biosurfactant) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene (Ba_K1688002) coding for the enzyme RhlA creates the fatty acid tail and the enzyme RhlB from rhlB gene connects the tail to a rhamnose molecule. The rhlB gene has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomonas aeruginosa. The biobrick Ba_K1688003 is an assembly of RBS with BBa_K1331004 that codes for RhlB enzyme


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
    Illegal NgoMIV site found at 778
    Illegal NgoMIV site found at 891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 407


[edit]
Categories
Parameters
n/aRhlB Gene