Translational_Unit

Part:BBa_K1688002

Designed by: Biosurfers   Group: iGEM15_Uppsala   (2015-09-15)

RhlA Gene

The enzymes responsible for producing mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The protein encoded by these two make up the subunits of rhamnosyltransferase I. The mono- rhamnolipids consist of a hydrophilic rhamnose head and a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail. The rhlA gene coding for the enzyme RhlA creates the fatty acid tail and has been lifted and improved by iGEM10 tokyo-NoKoGen and iGEM14 Nankai from Pseudomonas aeruginosa. The biobrick Ba_K1688002 is an assembly of RBS with BBa_K1331001.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 87
    Illegal BamHI site found at 647
    Illegal XhoI site found at 823
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 312
    Illegal BsaI.rc site found at 496


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