Coding

Part:BBa_K1683005

Designed by: Matthew W Mortensen   Group: iGEM15_WLC-Milwaukee   (2015-09-17)

E. coli tolC signaling sequence + Pseudomonas aeruginosa oprM

The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC homolog gene, oprM, from Pseudomonas aeruginosa PA01. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 102
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None