Part:BBa_K1682017

P_araBAD-GFP-P_lac-RFP

This composite part is consists of a L-arabinose inducible P_araBAD with GFP reporter BBa_I13540, and an IPTG inducible P_lac with RFP reporter Part:BBa_K1682016. The part was designed to investigate the possible effect of parallel sensing within a single plasmid.

Mechanism

Figure 1. Schematic diagram of the inducible P_araBAD-GFP-P_lac-mrfp construct. The system can sense L-arabinose to give GFP output or sense IPTG to give RFP output.

Usage and Biology

In order to investigate the effect of parallel sensing, the dose response of P_araBAD-gfp in pSB3K3-BBa_K1682017 (double construct) was compared with that in pSB3K3-BBa_I13540 (single construct) in E. coli DH10B.

Figure 2. Response curve of P_araBAD-gfp in a single construct and double construct under varying L-arabinose concentration.The different blue curves indicate different induction level of the co-expressed P_lac-mrfp construct. At varying the induction level of the co-expressed P_lac-mrfp construct seems to have an effect on the expression level of P_araBAD-gfp in a double construct. At a higher IPTG concentration, the maximum expression of P_araBAD-gfp decreases.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal NheI site found at 3185
  Illegal NheI site found at 3208
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 4386
  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 979
  Illegal AgeI site found at 2882
  Illegal AgeI site found at 2994
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1880
  Illegal SapI site found at 961