This composite part is consists of a L-arabinose inducible ParaBAD with GFP reporter BBa_I13540, and an IPTG inducible Plac with RFP reporter Part:BBa_K1682016. The part was designed to investigate the possible effect of parallel sensing within a single plasmid.
Figure 1. Schematic diagram of the inducible ParaBAD-GFP-Plac-mrfp construct. The system can sense L-arabinose to give GFP output or sense IPTG to give RFP output.
Usage and Biology
In order to investigate the effect of parallel sensing, the dose response of ParaBAD-gfp in pSB3K3-BBa_K1682017 (double construct) was compared with that in pSB3K3-BBa_I13540 (single construct) in E. coli DH10B.
Figure 2. Response curve of ParaBAD-gfp in a single construct and double construct under varying L-arabinose concentration.The different blue curves indicate different induction level of the co-expressed Plac-mrfp construct. At varying the induction level of the co-expressed Plac-mrfp construct seems to have an effect on the expression level of ParaBAD-gfp in a double construct. At a higher IPTG concentration, the maximum expression of ParaBAD-gfp decreases.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
Illegal NheI site found at 3185
Illegal NheI site found at 3208
- 21Illegal BglII site found at 4386
Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
Illegal AgeI site found at 2882
Illegal AgeI site found at 2994
- 1000Illegal BsaI.rc site found at 1880