Project

Part:BBa_K1681002

Designed by: Sameer Andani   Group: iGEM15_UIUC_Illinois   (2015-09-18)
    <img src="800px-UIUC_Illinois_PartD_Updated.png">
  • Part Name: PL lac O-1 SCRIBE(BsaI)
  • Short Description: IPTG-inducible promoter and BsaI SCRIBE cassette
  • Long Description: This part is the IPTG-inducible promoter PL lac O-1 immediately upstream of SCRIBE(BsaI), with no scar site in between.
  • Design Considerations: This part was designed to be used as a positive control for testing SCRIBE cassettes with different promoters after using Golden Gate assembly to add custom DNA segments into the msd region for site-specific recombination. See Bba_K1681000 for a more detailed description as to how such custom DNA segments would be inserted into the host’s genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1193
    Illegal XhoI site found at 435
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 183
    Illegal BsaI.rc site found at 177


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Parameters
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