Part:BBa_K1681001

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• Part Name: SCRIBE(BsaI)
• Short Description: SCRIBE cassette with BsaI sites for Golden Gate assembly
• Long Description: SCRIBE(BsaI) is a SCRIBE cassette including two restriction sites of the Type-II restriction enzyme BsaI in the msd region of the retron. This part is compatible with Golden Gate assembly.
• Design Considerations: This part was designed as a modular assembly part for future users to add custom DNA segments into the msd region for site-specific recombination via Golden Gate assembly and to add promoters of their choice via Standard or 3A assembly. See Bba_K1681000 for a more detailed description as to how such custom DNA segments would be inserted into the host’s genome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1119
  Illegal XhoI site found at 361
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 106
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 109
  Illegal BsaI.rc site found at 103