Device

Part:BBa_K1679040

Designed by: Xihan Zhang   Group: iGEM15_OUC-China   (2015-09-17)

pT7-100 + RiboJ + GFP + LVA + 3 terminnators

This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. The four T7 RNA polymerases are a family with different activity, the T7 RNA polymerase in this device have approximate relative activity of 100%.


Usage and Biology

PT7.jpg
Fig.1 T7 protein expression device.
4pT7.jpg
Fig.2 The 4 T7 RNA polymerase and their approximate relative activity.[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//direction/reverse
Parameters
None