Part:BBa_K1679039

pT7-10 + RiboJ + GFP + LVA + 3 terminnators

This device is design for protein expression and promoter measurement. It constructed with a T7 RNA polymerase which has other 3 mutations, a RiboJ, a green fluorescent protein, a LVA degradation tag, a T7 terminator, a rrnB T1 terminator and a rrnB T2 terminator. The four T7 RNA polymerases are a family with different activity, the T7 RNA polymerase in this device have approximate relative activity of 10%.

Usage and Biology

Fig.1 T7 protein expression device.

Fig.2 The 4 T7 RNA polymerase and their approximate relative activity.[1]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1019
  
• 1000
  COMPATIBLE WITH RFC[1000]