Coding

Part:BBa_K1679037

Designed by: Jinyang Liang   Group: iGEM15_OUC-China   (2015-09-17)


VMA Dead, an inactivated intein

We have submit VMA, a intein which can self-splice at 18℃, means that it can catalyze its own excision from the flanking polypeptides, or exteins, as well as ligation of the exteins. VMA Dead is an inactivated mutation of VMA, we constructed it by replace Asn-464 with Gln. We design this part as a control group of VMA.We constructed this part from VMA Dead and its exteins in original host.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None