Part:BBa_K1679036

pT7-100

The four T7 RNA polymerases are a family with different activity, this T7 RNA polymerase is the wild type which has three low-activity mutations. We design it as a part of our T7 protein expression device, which is high level and need low-activity promoter to exclude bacterial stress responses. Users can replace the T7 promoter with it or other promoters through the restriction site we added.

Usage and Biology

Fig.1 The 4 T7 RNA polymerase and their approximate relative activity.[1]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]