Part:BBa_K1679033

pT7-4

The four T7 RNA polymerases are a family with different activity, this T7 RNA polymerase have approximate relative activity of 4% compare to the wild type pT7. We design it as a part of our T7 protein expression device, which is high level and need low-activity promoter to exclude bacterial stress responses. Users can replace the T7 promoter with it or other promoters through the restriction site we added.

Usage and Biology

The 4 T7 RNA polymerase and their approximate relative activity.

Experiment

We expressed GFP on this device in BL21(DE3). Three monoclonal colonies were selected and culture until OD600 0.5(37℃, 220rpm).We calculated the Fluorescence(excitation wavelength-584 nm and emission wavelength-607 nm) and OD600.

Fig.1 Three colonies with this part and three colonies withBBa_K1679026 were selected to measure.

Fig.2 The fluorescent/OD of three colonies in fig.1 were averaged.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]