Coding

Part:BBa_K1679022

Designed by: Xihan Zhang   Group: iGEM15_OUC-China   (2015-09-17)


KIG, kcoil+intein+gfp

KIG is a fusion protein constructed from Kcoil, VMA and green fluorescent protein. Kcoil is a synthetic coiled-coil domain designed specifically to bind to Ecoil with high affinity and specificity. VMA is a thermosensitive intein. We design it to use cooperatively with BBa_K1189018(2013,Calgary).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 31
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None