Regulatory

Part:BBa_K1679004

Designed by: Qikai Qin   Group: iGEM15_OUC-China   (2015-09-16)

J23101 and RiboJ

BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme with an additional 23-nt hairpin immediately downstream to help expose the RBS. This part can be used as a promoter-like part.

Attention Please!

The right sequence should be "tttacagctagctcagtcctaggtattatgctagctactagagagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaa". But the DNA Sample we submit is correct. We are so sorry for this problem.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter/anderson
Parameters
positive_regulators