Coding

Part:BBa_K1678003

Designed by: Barthelemy Caron   Group: iGEM15_Paris_Bettencourt   (2015-09-16)

RibT promoted by p48 synthetic promoter

Bacillus subtilis RibT gene for riboflavin production. RibT code for the Beta-chain of the riboflavin synthase.

Coding sequence has been codon optimized for Lactobacillus plantarum.

Protein is expressed by a L.plantarum synthetic promoter p48 <a href="https://parts.igem.org/Part:BBa_K1678004:Experience">BBa_K1678004</a>.

The gene is terminated by the Tldh terminator (Spath et al. Microbial Cell Factories 2012, 11:141).

The RBS and the spacing between the RBS and the coding sequence are optimized for Lactobacillus plantarum (Tauer et al. Microbial Cell Factories 2014, 13:150).

The expressed protein catalyses one step in the riboflavin pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 21
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 552


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Parameters
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