Plasmid_Backbone
Part:BBa_K1677202
Designed by: Mackenzie Labine-Romain Group: iGEM15_BABS_UNSW_Australia (2015-09-15)
psbA2
This integrative plasmid facilitates transformation of Synechocystis PCC6803 and similar species. It inserts gene of choice into the Synechocystis psbA2 gene by homologous recombination.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal XbaI site found at 987 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal EcoRI site found at 4865
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 28
Illegal NotI site found at 4871 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal EcoRI site found at 4865
Illegal BglII site found at 22
Illegal BamHI site found at 839
Illegal XhoI site found at 4201 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4865
Illegal suffix found at 2
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal XbaI site found at 987 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4865
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal XbaI site found at 987
Illegal XbaI site found at 4880
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1038
Illegal NgoMIV site found at 3864 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2688
Illegal SapI site found at 1605
[edit]
Categories
Parameters
None |