Coding

Part:BBa_K1677102

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-08-20)


Invasin with Usp45 Signal Peptide (Lactococcus)

Invasin enables bacteria to invade mammalian cells via beta-integrin mediated endocytosis. Invasin was codon optimised for lactococcus species, and the lactococcal Usp45 signal peptide was added to localise it to the outermembrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 794
    Illegal BglII site found at 878
    Illegal BglII site found at 2318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2347
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None