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Coding

Part:BBa_K1677100

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-08-06)

Listeriolysin (lactococcus)

Listeriolysin O (LLO) is a hemolysin that forms pores in phagosomes, and allows the bacterium expressing it to escape into the cytoplasm without damaging the plasma membrane.

This part was adapted from BBa_K299812 (Listeriolysin) and codon optimised for Lactococcus species.

THIS IS THE WRONG SEQUENCE IGNORE THIS PART!!! THE CORRECT ONE IS BBa_K1677101

WHY CAN WE NOT DELETE PARTS

IT IS HUMAN TO ERR

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1621
    Illegal PstI site found at 1635
    Illegal NotI site found at 7
    Illegal NotI site found at 1628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1621
    Illegal PstI site found at 1635
  • 1000
    COMPATIBLE WITH RFC[1000]


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