Composite

Part:BBa_K1676098

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)

Mutant 1 of Lactate Permease Expression Part

This construct is an operon for expression of our mutant LDP with pLac as a promoter.

We have charecterised the growth of Shewanella oneidensis MR1 with this construct.

Below is the growth curve of Shewanella containing this construct.

SH8_mutants.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1019


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Parameters
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