Composite

Part:BBa_K1676085

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)

Mutant 9 of LDH and wildtype LDP

This construct is an operon for expression of our mutant LDH and wildtype LDP.

The is a mutant of lactate dehydrogenase which contain mutation to these positions: S170P, R180G, I240N, H275Q, P72Q, A76V.

Mfcreading.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2191


[edit]
Categories
Parameters
None