Part:BBa_K1675009
a device to produce alkali and neutralize the external acidic environment
This device is part of our basic regulation circuit in 2015 project of BIT-China.
Using an acid induced promoter asr(BBa_K1170000), strong RBS(BBa_B0034),alkaline substance producing gene gadA coding region(BBa_K1675003).Will produce alkali under acidic environment and neutralize the external pH.
When the initial state of fermentation is under the acidic stress, the regulation capacity of test group is higherthan the control group. (Shown in Fig.1 and Fig.2)
Fig.1 The final fermentation result of the basic regulation system under acidic stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.
Fig.2 The growth curve of E.coli with basic regulation circuit under acidic stress.
When the initial state is under the alkaline stress, the response of the test group is quicker than the control group, and the capacity is a littlie higer than the control group. (Shown in Fig.3 and Fig.4)
Fig.3 The final fermentation result of the basic regulation system under alkaline stress. By calculating the pH change of unit active biomass in unit time, This figure can be drawed.
Fig.4 The growth curve of E.coli with basic regulation circuit under alkaline stress.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 844
Illegal AgeI site found at 904 - 1000COMPATIBLE WITH RFC[1000]
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