Composite

Part:BBa_K1675005

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-10)


J23119+B0034+NhaB(BBa_K1675001)

The constitutive promoter J23119, the ribosome binding site B0034 and the functional gene nhaB were used in this new standard part. This part is available for the bacteria to survive in alkaline environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1064
    Illegal AgeI site found at 1376
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/pump
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli str. K-12 substr.