Composite

Part:BBa_K1674005

Designed by: Roni Cohen   Group: iGEM15_Technion_Israel   (2015-09-15)

pT7 - ZWF

 

This part was created for checking the activity of the basic part zwf (BBa_K1674004). As part of our project we tried to enhance NADPH in order to provide our 3αHSD enzyme a proper environment with excess of NADPH. Thus we clone the zwf gene into pSB1C3 with T7 promoter (BBa_K525998) to obtain over-expression of the zwf . For experiments and results see the main page of the zwf BioBrick.

 

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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